Integrated NIRS and QTL assays reveal minor mannose and galactose as contrast lignocellulose factors for biomass enzymatic saccharification in rice

Abstract Background Identifying lignocellulose recalcitrant factors and exploring their genetic properties are essential for enhanced biomass enzymatic saccharification in bioenergy crops. Despite modification of major wall polymers has been implemented reduced recalcitrance engineered crops, it could most cause a penalty plant growth yield. Alternatively, is increasingly considered to improve minor components, but an applicable approach required efficient assay large population samples. Hence, this study collected total 100 rice straw samples characterized all monosaccharides by integrating NIRS modeling QTL profiling. Results By performing classic chemical analyses establishing optimal equations, examined four (acid-soluble lignin/ASL, acid-insoluble lignin/AIL, three lignin monomers, crystalline cellulose), which led largely varied hexoses yields achieved from hydrolyses after two alkali pretreatments were conducted with straws. Correlation indicated that mannose galactose can play contrast role at P < 0.0 l level ( n = 100). Meanwhile, we found the QTLs controlling mannose, galactose, lignin-related traits, co-located. combining maps, further interpreted mannose-rich hemicellulose may assist AIL disassociation saccharification, whereas galactose-rich polysaccharides should be effectively extracted ASL pretreatment condensed association cellulose microfibrils. Conclusions profiling samples, identified content positively affect while had significantly negative impact. It also sorted out distinctively associate deposition network construction. demonstrates fast assessments rice, providing potential strategy crop breeding processing.